T Williams Rhos.

Our results indicate two of the Hsf1 mutant receptors signal independently of added CK in yeast, and all three have increased binding affinities for the natural CKs tested (Figure 1E; Table 1). Hsf1-AEWL arose via EMS mutagenesis of the inbred A619 and Hsf1-7322 via EMS mutagenesis of the inbred A632 in independent screens.

M.G.M., S.C., H.S., G.A.R., B.L., and N.B. For hormone treatments, 20 imbibed kernels per replicate were placed embryo-side down on two paper towels in a Petri dish, covered with two more layers of paper towel, and filled with 15 mL of CK treatment or the water control solution. Measurement of adult plant traits of the three Hsf1 mutant alleles was performed on field-grown families segregating 50% wild type:50% Hsf1 heterozygotes. This family segregated 9 wild type, 12 abph1/abph1, 10 Hsf1-1603/+, and 15 double Hsf1-1603/+ abph1/abph1, which fits a 1:1:1:1 expected ratio. Grass leaves display a prominent P-D polarity consisting of a proximal sheath separated from the distal blade by the auricle and ligule. (D) Macrohair production on the abaxial sheath and auricle (white arrowheads) of 2-week-old B73 water-treated (– CK) and 10 µM BA-treated (+ CK) seedlings. Competitive CK binding assays were performed in duplicate or triplicate as described previously (Lomin et al., 2011; Donaldson et al., 2019). ΔCt of mutant (Hsf1) and ΔCt of the wild type was expressed as the difference in cycle threshold (Ct) value between target gene and the endogenous control. How increased CK signaling impacts these growth zones to determine final leaf size will require further analysis building upon these previous studies.

Three HPTs, three type-B RRs, and seven type-A RRs (ZmRRs) have also been identified in maize (Asakura et al., 2003). To test this idea, we performed 6-d treatments on segregating Hsf1-1603/+ seeds using 0.1 μM CK, a concentration that did not elicit leaf size changes in B73 inbred seed (Supplemental Figure 6A). Tech Tips Online.

Supplemental Figure 5.

Supplemental Figure 4.

Hsf1 increased macrohair production not only on the abaxial sheath, adaxial blade, auricle, and blade margins of adult leaves but also on juvenile and transitional leaves, which are typically glabrous. Crosville home. Biochemical analysis and structural modeling suggest the mutated residues near the CK binding pocket affect CK binding affinity. To gain a better understanding of the signaling mechanisms that mediate leaf pattern specification, we initiated a study of the semidominant Hairy Sheath Frayed1 (Hsf1) mutation that alters P-D leaf development in maize (Bertrand-Garcia and Freeling, 1991a). Copyright 2018 Hutchison. Hutchison Vale FC The Club is looking for coaches and players for their Tots and Kids Schools and their soccer 7’s ages born 2012-2011-2010-2009-2008-2007s and their 11 a – side teams. [PMC free article] [Google Scholar] Chen T, Yong R, Dong H, Duncan MJ.

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