Cell Transplant. doi: 10.1002/jcp.21081, Lennon, D. P., Haynesworth, S. E., Bruder, S. P., Jaiswal, N., and Caplan, A. I. ASCs expanded in PRP maintain a classic immunophenotype and morphology, and PRP increases proliferation when compared to FBS (Kocaoemer et al., 2007; Chieregato et al., 2011; Atashi et al., 2015). FBS contains growth factors (GFs) and other elements essential for ASC attachment, expansion, maintenance, and proliferation in vitro (Lennon et al., 1995, 1996; Zuk et al., 2001; van der Valk et al., 2010). Biol., 17 October 2016 Most studies comparing different culture media used the criteria specified by the ISCT and IFATS to validate the use of an alternative to FBS. Alternatively, allogeneic HS can be pooled, yielding larger quantities for laboratory experimentation and can undergo rigorous quality testing by a blood bank (e.g., testing for the absence of infectious agents and contamination with other blood cells) prior to use in humans (Bieback et al., 2009). 5:e1567. 5, 1–10.
The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. The selection of medium supplementation will depend on the downstream application of the ASCs and their efficacy and safety in preclinical studies. After whole blood has been allowed to clot in the absence of an anticoagulant and has been centrifuged, serum is the resulting liquid portion that does not contain platelets, white blood cells or red blood cells (Figure 1; Stedman, 2006). (2011). Culture effects of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on cryopreserved human adipose- derived stromal/stem cell proliferation and adipogenesis. Most of these studies examine ASC morphology, proliferation, immunophenotype, and the ability of these cells to differentiate along osteogenic, chondrogenic, and adipogenic lineages in different culture media. Commercially available XF medium is expensive and preparing in-house XF medium can be time-consuming and may increase the risk of batch-to-batch variation (Lund et al., 2009; Baer et al., 2010; Rajala et al., 2010; Yang et al., 2012; Kyllonen et al., 2013; Patrikoski et al., 2013; Oikonomopoulos et al., 2015). In vitro Cell. Med. Gastroenterology 136, 978–989. Ahearne, M., Lysaght, J., and Lynch, A. P. (2014). Often SF media are supplemented with serum albumin.

J. (2013). They have also recommended that surface marker expression should be measured by multi-color antibody staining (Bourin et al., 2013).


Serum is produced when whole blood is allowed to clot and centrifuged to pellet red and white blood cells, and platelets. Stem Cell Res. Int. doi: 10.1016/j.jcyt.2013.02.006, Busser, H., Najar, M., Raicevic, G., Pieters, K., Velez Pombo, R., Philippart, P., et al. The resulting PPP is stored at 4°C and is referred to as fresh plasma. 49, 152–156.

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Ther. Gibco FBS can provide the cell health, maintenance and viability critical for optimal performance and cell growth in your culture medium.

How do we choose culture medium type; DMEM,MEM,RPMI? (2010). PRP is produced by separating plasma from red blood cells at slower centrifugation speeds, which prevents the pelleting of the platelets (Figure 1). Moreover, by pooling many donors, a large quantity can be obtained for supplementation, which makes HPL preferable to PRP (Kocaoemer et al., 2007; Bieback et al., 2009; Chieregato et al., 2011). J. doi: 10.1042/BSR20150067, Bernardo, M. E., Avanzini, M. A., Perotti, C., Cometa, A. M., Moretta, A., Lenta, E., et al. activity is 5 times than that for FBS. Stute, N., Holtz, K., Bubenheim, M., Lange, C., Blake, F., and Zander, A. R. (2004). 40, 1135–1139. Toxicol. School 79, 444–452. Atherosclerosis 240, 121–124. However, another study observed a negative effect on adipogenic and osteogenic differentiation in long term cultured ASCs (Gharibi and Hughes, 2012). No use, distribution or reproduction is permitted which does not comply with these terms.

Other than that, both serums provide the same substances necessary for cell culture. While FBS is used traditionally in the research setting for in vitro expansion, it does carry the risk of xenoimmunization and zoonotic transmission when used for expanding cells destined for therapeutic purposes.

Human and animal mesenchymal progenitor cells from bone marrow: identification of serum for optimal selection and proliferation.

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